Several Hepatitis C drugs have been found to inhibit a crucial protein enzyme in the novel coronavirus SARS-CoV-2. The experiments were led by researchers at the US Department of Energy’s Oak Ridge National Laboratory. The results are published in the journal structure.
Once it has entered the human cell using its spike protein, the coronavirus uses an enzyme called the main protease to replicate. The coronavirus expresses long chains of compounds, which must be cut by the main protease to become functional proteins. Inhibiting the functioning of the main protease, therefore, is vital to stopping the virus from spreading in patients with Covid-19.
The new study is part of efforts to quickly develop treatments for Covid-19 by repurposing existing drugs for other viral diseases.
Researchers looked at several well-known drug molecules for potential repurposing efforts. These included three hepatitis C protease inhibitors: telaprevir, narlaprevir and boceprevir.
The team performed room-temperature X-ray measurements to build a three-dimensional map of chemical bonds formed between the protease and the drug molecules. The experiments yielded promising results for certain hepatitis C drugs in their ability to bind and inhibit the main protease — particularly boceprevir and narlaprevir. 📣 Express Explained is now on Telegram
The study used a technique called in vitro enzyme kinetics. This enables researchers to study the protease and the inhibitor in a test tube to measure the inhibitor’s binding affinity with the protease. The higher the binding affinity, the more effective the inhibitor is at blocking the protease from functioning.
The study also sheds light on a peculiar behaviour of the protease’s ability to change or adapt its shape according to the size and structure of the inhibitor molecule it binds to. Pockets within the protease where a drug molecule would attach are highly flexible, and can either open or close to an extent.
Source: Oak Ridge National Laboratory, US Department of Energyx
📣 The Indian Express is now on Telegram. Click here to join our channel (@indianexpress) and stay updated with the latest headlines