Rai, lodged separately in Thane central prison, had not been produced before the court since March 31.
Indrani has been lodged in the Byculla women’s prison and the other two male accused in the case, Indrani’s former husbands, Sanjeev Khanna and Peter Mukerjea, are lodged at the Arthur Road jail.
The other two are psychiatrist Dr Yusuf Matcheswala and Indrani’s secretary Kajal Sharma.
The CBI told the court that it wants to gather more evidence and investigate if other people were involved in the conspiracy.
CBI has alleged that Peter was actively involved in the conspiracy to murder Sheena which was hatched by Indrani, her second husband Sanjiv Khanna and driver Shyamvar Rai who all have been arrested by the agency.
“Both, Peter and Indrani were unhappy with the relationship between Rahul and Sheena,” said Additional Solicitor General of India Anil Singh.
The special CBI judge H S Mahajan on Saturday extended the judicial custody of Indrani, Khanna and her former driver Shyam Rai till February 12.
Peter Mukerjea, who has been arrested in connection with the Sheena Bora murder case, was grilled for several hours by CBI sleuths on Friday.
Conversations in calls recorded by Rahul Mukerjea helped CBI build its case against Peter Mukerjea, who was at one end of the conference calls.
Sheena Bora was allegedly strangled in a car and then her body was burnt and dumped in a forest in Raigad, about 84 km from Mumbai.
Agency will use a map as imagery for the first time to give ‘accurate’ distances and perspective into the paths the victim and the accused took.
The court also extended judicial custody of Mukerjea, Khanna and Rai until November 20.
CBI had last week moved an application before the court seeking Indrani Mukerjea’s voice samples for verification in the murder investigation of her daughter Sheena Bora.
Indrani Mukerjea was shifted to JJ hospital for treatment.
Rai, a former driver employed with jailed media executive Indrani Mukerjea, had submitted a handwritten letter in Hindi to the court on October 23, in which he stated that he wanted to tell the truth about the case.